Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

F6057

Sigma-Aldrich

Fluoroshield™ with DAPI

liquid

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

product name

Fluoroshield™ with DAPI, histology mounting medium

Niveau de qualité

200

Forme

liquid

Composition

Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl

Technique(s)

immunofluorescence: suitable

Plage de pH

7.9-8.3

Solubilité

water: soluble

Fluorescence

λex 360 nm; λem 460 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Catégories apparentées

Description générale

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield™ with DAPI has been used:
  • as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
  • as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
  • as mounting solution for immunostained heart sections for nuclei detection

Informations légales

FluoroShield is a trademark of ImmunoBioScience Corp.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker
miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Nascimento DS, et al.
Stem Cell Research & Therapy, 5(1), 5-5 (2014)
Afficher tous les articles scientifiques apparentés

Articles

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique