F3174

Fpg Protein from Escherichia coli

≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

• Synonyme(s) : DNA-(apurinic or apyrimidinic site)lyase MutM (APlyase MutM), Fapy-DNAglycosylase, Formamidopyrimidine-DNA glycosylase, Fpg Protein from Escherichia coli, Recombinant, Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM
• Numéro CAS: 78783-53-6
• Numéro de classification (Commission des enzymes): 3.2.2.23 (BRENDA, IUBMB)
• Numéro MDL: MFCD02264758
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: >20,000 units/mg protein
• Poids mol.: 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
• Composition: protein, 0.1- 0.3 mg/mL Bradford
• Conditions de stockage: (Tightly closed)
• Technique(s): nucleic acid detection: suitable
• Numéro d'accès UniProt: P05523
• Application(s): genomic analysis
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: Escherichia coli CFT073 ... mutM(1038243) ; Escherichia coli K12 ... mutM(946765)

Description

Description générale

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

Fpg contains two domains separated by a flexible hinge.

Research area: Cell signaling

Application

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Actions biochimiques/physiologiques

Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.

Fpg specifically acts on 3′- and 5′-phosphodiester bonds.

Définition de l'unité

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.

Forme physique

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)