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Principaux documents

E3024

Sigma-Aldrich

Earle′s Balanced Salts

With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

EBSS

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)

Conditions d'expédition

ambient

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Description générale

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).

Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.

Autres remarques

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Morgan Wong et al.
American journal of physiology. Cell physiology, 316(5), C753-C765 (2019-03-21)
A defective tight junction (TJ) barrier is a key pathogenic factor for inflammatory bowel disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy-related protein-6 (ATG6/beclin 1), a key protein in the
Stanislas Morand et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(4), 1205-1218 (2008-12-17)
Dual oxidases (Duox1 and Duox2) are plasma membrane-targeted hydrogen peroxide generators that support extracellular hemoperoxidases. Duox activator 2 (Duoxa2), initially described as an endoplasmic reticulum resident protein, functions as a maturation factor needed to deliver active Duox2 to the cell
Natthakan Thongon et al.
Cancer & metabolism, 6, 1-1 (2018-03-16)
Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in NAD+ biosynthesis from nicotinamide, exhibit anticancer effects in preclinical models. However, continuous exposure to NAMPT inhibitors, such as FK866, can induce acquired resistance. We developed FK866-resistant CCRF-CEM (T cell acute lymphoblastic
Léon-Charles Tranchevent et al.
Genome research, 27(6), 1087-1097 (2017-04-20)
Transcriptomic genome-wide analyses demonstrate massive variation of alternative splicing in many physiological and pathological situations. One major challenge is now to establish the biological contribution of alternative splicing variation in physiological- or pathological-associated cellular phenotypes. Toward this end, we developed
Alexandra Bunda et al.
FEBS open bio, 9(9), 1603-1616 (2019-07-18)
Presynaptic CaV 2.2 (N-type) channels are fundamental for transmitter release across the nervous system. The gene encoding CaV 2.2 channels, Cacna1b, contains alternatively spliced exons that result in functionally distinct splice variants (e18a, e24a, e31a, and 37a/37b). Alternative splicing of

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