E2264

Endoglycosidase F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

• Synonyme(s) : Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
• Numéro de classification (Commission des enzymes): 3.2.1.96 (BRENDA, IUBMB)
• Numéro MDL: MFCD00146843
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Conjugué: (N-linked)
• Forme: solution
• Activité spécifique: 30 U/mg
• Poids mol.: 32 kDa
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Actions biochimiques/physiologiques

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Conditionnement

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Définition de l'unité

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Forme physique

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)