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Key Documents

DNTP100A

Sigma-Aldrich

Deoxynucleotide Set, 100 mM

Individual dNTPs for routine PCR; 1 mL each

Synonyme(s) :

PCR dNTPs, dNTP set, dNTPs

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About This Item

Code UNSPSC :
41106305
Nomenclature NACRES :
NA.52

Forme

liquid

Concentration

100 mM

Couleur

colorless

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Deoxynucleotide triphosphates (dNTPs) are negatively charged organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 1.0 mL each of 100mM aqueous solutions of four deoxyribonucleotides namely deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.

Application

Deoxynucleotide set, 100 mM has been used in:
  • the amplification of DNA in polymerase chain reaction (PCR)
  • quantitative PCR (qPCR)
  • multiplex PCR

Actions biochimiques/physiologiques

Deoxynucleotide triphosphates (dNTPs) are building blocks of nucleic acid molecules and are acidic in nature. They act as substrates for DNA polymerizing enzymes. dNTPs are essential components of PCR master mixes as it is necessary for DNA amplification reaction. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.

Caractéristiques et avantages

  • Provided as 4 separate tubes of dNTPs
  • Allows optimization of individual dNTP concentrations

Conditionnement

1.0 mL each of 100 mM dATP, dCTP, dGTP and dTTP

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Aleksandr M Agafontsev et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 25(11), 2684-2694 (2018-10-06)
Nucleotides are constituents of nucleic acids and they have a variety of functions in cellular metabolism. Synthetic receptors and sensors are required to reveal the role of nucleotides in living organisms and mechanisms of signal transduction events. In recent years
Developmental potential of vitrified Holstein cattle embryos fertilized in vitro with sex-sorted sperm
Xu J, et al.
Journal of Dairy Science, 89(7), 2510-2518 (2006)
Establishment of a Fusarium graminearum Infection Model in Arabidopsis thaliana Leaves and Floral Tissues
Nalam, et al.
Bio-protocol, 6 (2016)
Magnesium is a critical element for competent development of bovine embryos
An L, et al.
Theriogenology, 140, 109-116 (2019)
Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples
Cad?rc?, et al.
Turkish Journal of Agriculture - Food Science and Technology null

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