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Key Documents

D9905

Sigma-Aldrich

Denhardt′s Solution

for Northern and Southern blotting, lyophilized powder

Synonyme(s) :

Denhardt′s Solution For Blotting Technique

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

100

Forme

lyophilized powder

Technique(s)

Southern blotting: suitable

Activité étrangère

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Température de stockage

2-8°C

Description générale

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Denhardt′s Solution has been used:
  • as a component in hybridization buffer for the hybridization of brain sections
  • in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
  • in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Actions biochimiques/physiologiques

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

Composants

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

Notes préparatoires

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
M Simpson-Holley et al.
Journal of virology, 85(4), 1581-1593 (2010-12-15)
Vaccinia virus (VV) mutants lacking the double-stranded RNA (dsRNA)-binding E3L protein (ΔE3L mutant VV) show restricted replication in most cell types, as dsRNA produced by VV activates protein kinase R (PKR), leading to eIF2α phosphorylation and impaired translation initiation. Here
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
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