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D6063

Sigma-Aldrich

REDTaq® SuperPak™ DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Synonyme(s) :

DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 250 reactions

Caractéristiques

dNTPs included
hotstart: no

Concentration

1 unit/μL

Technique(s)

PCR: suitable

Couleur

red

Entrée

purified DNA

Adéquation

suitable for PCR

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

REDTaq® SuperPak™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Application

REDTaq® SuperPak™ DNA Polymerase has been used in:
  • reverse transcriptase-polymerase chain reaction (RT-PCR)
  • nested PCR
  • routine PCR amplification

Caractéristiques et avantages

  • Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
  • Suitable for nested PCR
  • Contains ultrapure 10mM dNTP mix in a separate vial

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 includedFDS
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A Jóźwik et al.
Veterinary research communications, 29(4), 347-359 (2005-03-09)
Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
M Yeste-Velasco et al.
Neuroscience, 159(3), 1135-1147 (2009-04-10)
Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
D Alvira et al.
Neuroscience, 147(3), 746-756 (2007-06-23)
The mechanism involved in neuronal apoptosis is largely unknown. Studies performed on neuronal cell cultures provide information about the pathways which orchestrate the process of neuronal loss and potential drugs for the treatment of neurological disorders. In the present study
Vishnu Chintalgattu et al.
Journal of molecular and cellular cardiology, 35(3), 277-286 (2003-04-05)
Vascular endothelial growth factor (VEGF), produced predominantly by endothelial cells, is involved in angiogenesis and mitogenesis. Myofibroblasts (myoFb) are phenotypically transformed fibroblast-like cells found at the site of myocardial infarction. Since myoFb play a role in tissue repair/remodeling at the
M Yeste-Velasco et al.
Neuropharmacology, 53(2), 295-307 (2007-07-07)
Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
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Protocoles

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

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