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Key Documents

D1145

Sigma-Aldrich

DMEM - high glucose

With sodium bicarbonate, without ʟ-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell cutlure

Synonyme(s) :

DME, DMEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

product name

Milieu d'Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Application

Le milieu de Eagle modifié par Dulbecco (DMEM) est une variante du milieu de Eagle de base (BME) qui contient quatre fois plus d'acides aminés et de vitamines. La formulation d'origine contenait 1 000 mg/l de glucose et servait à la culture de cellules embryonnaires de souris. Depuis, le milieu a été modifié à plusieurs reprises pour convenir aux primocultures de cellules de souris et de poulet ainsi que de diverses cellules normales ou transformées. Chacun de ces milieux apporte une combinaison différente de L-glutamine et de pyruvate de sodium. En outre, le taux de glucose a été augmenté à 4 500 mg/l, d'où le nom "DMEM/High" pour DMEM riche en glucose.

Reconstitution

À supplémenter avec 0,584 g/l de L-glutamine.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Tracy A O'Mara et al.
Nature communications, 9(1), 3166-3166 (2018-08-11)
Endometrial cancer is the most commonly diagnosed cancer of the female reproductive tract in developed countries. Through genome-wide association studies (GWAS), we have previously identified eight risk loci for endometrial cancer. Here, we present an expanded meta-analysis of 12,906 endometrial
Ştefan Ţălu et al.
Molecular vision, 24, 902-912 (2019-02-05)
This study aimed to quantify the three-dimensional micromorphology of the surface of the human lens capsule as a function of age. Imaging experiments were conducted on whole human lenses received from eight human cadavers (donor age range: 30-88 years). Imaging
Jikai Zhao et al.
The Journal of steroid biochemistry and molecular biology, 178, 354-368 (2018-02-17)
Hippocampal synaptic plasticity is the basis of spatial memory and cognition and is strongly regulated by both testicular androgens (testosterone, T) and hippocampal estrogens (17β-estradiol, E2) converted from T by aromatase, which is inhibited by letrozole (LET), but the contribution
Sina Naddaf Dezfuli et al.
Materials science & engineering. C, Materials for biological applications, 79, 647-660 (2017-06-21)
The present research was aimed at developing magnesium-matrix composites that could allow effective control over their physiochemical and mechanical responses when in contact with physiological solutions. A biodegradable, bioactive ceramic - bredigite was chosen as the reinforcing phase in the
Shinsuke Matsuzaki et al.
PloS one, 10(4), e0123578-e0123578 (2015-05-01)
Recently, accumulating reports have suggested the importance of endoplasmic reticulum (ER) stress signaling in the differentiation of several tissues and cells, including myoblasts and osteoblasts. Secretory cells are easily subjected to ER stress during maturation of their secreted proteins. Skin

Protocoles

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Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Contenu apparenté

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

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