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CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

Numéro CE :
217-896-6
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Utilisation

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Technique(s)

flow cytometry: suitable
protein staining: suitable

Fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Caractéristiques et avantages

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principe

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Remarque sur l'analyse

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Composants de kit seuls

Réf. du produit
Description
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H300 + H310

Classification des risques

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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