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Sigma-Aldrich

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

Synonyme(s) :

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Technique(s)

centrifugation: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Application

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

Caractéristiques et avantages

  • Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
  • Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
  • Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
  • Convenient format - Saves time and reduces waste

Adéquation

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • Chloroplast Isolation Buffer 5x (CIB) 500 mL
  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 gFDS
  • Chloroplast Isolation Buffer 5x (CIB) 4 ea
  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mLFDS

Code de la classe de stockage

10 - Combustible liquids

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Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
The Complete Chloroplast Genome of 17 Individuals of Pest Species <I>Jacobaea vulgaris</I>: SNPs, Microsatellites and Barcoding Markers for Population and Phylogenetic Studies.
Doorduin, L., et al.
DNA Res., 1-13 (2011)
Esau Megias et al.
The protein journal, 37(3), 290-299 (2018-05-29)
Tymovirus is a genus of plant pathogenic viruses that infects several dicotyledonous plants worldwide, causing serious diseases in economically important crops. The known cytopathic effect on the host cell organelles involves chloroplast membrane deformation and the induction of vesicles in
Jimena Cascales et al.
Life (Basel, Switzerland), 7(4) (2017-11-23)
The use of molecular markers with inadequate variation levels has resulted in poorly resolved phylogenetic relationships within Ilex. Focusing on southern South American and Asian species, we aimed at contributing informative plastid markers. Also, we intended to gain insights into
Yanzhong Luo et al.
Frontiers in plant science, 11, 613-613 (2020-06-13)
The transportation of proteins encoded by nuclear genes from plant cytosol to chloroplast is essential for chloroplast functions. Proteins that have a chloroplast transit peptide (cTP) are imported into chloroplasts via translocases on the outer and inner chloroplast envelope. How
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