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Key Documents

CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Synonyme(s) :

BL21 strain

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.85

product name

BL21(DE3) Electrocompetent Cells, for protein expression

Source biologique

Escherichia coli

Qualité

for molecular biology

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Caractéristiques et avantages

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Composants

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Code de la classe de stockage

10 - Combustible liquids

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Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
Brandon R Lowe et al.
eLife, 10 (2021-02-02)
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
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Protocoles

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

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