JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: JVM-2, 96090515
• Source biologique: human unknown/unspecified
• Mode de croissance: Suspension
• Caryotype: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Description de la lignée cellulaire

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Application

Ig expression of neoplastic cells

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

Milieu de culture

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Autres remarques

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