CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: CFPAC-1, 91112501
• Source biologique: human pancreas (liver metastasis)
• Mode de croissance: Adherent
• Caryotype: Hyperdiploid, (65-75 chromosomes)
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Cystic fibrosis trans-membrane regulator (CFTR)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian pancreatic adenocarcinoma

Description de la lignée cellulaire

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

Application

Cystic fibrosis studies, genetic analysis

Milieu de culture

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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