HT29 Cell Line human

91072201, human colon, Epithelial

• Synonyme(s) : HT29 Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• Source biologique: human colon
• Mode de croissance: Adherent
• Caryotype: 2n = 46, hypertriploid
• Morphologie: Epithelial
• Produits: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Application

HT29 Cell Line has been used to determine viral titres of human parechovirus.[1] It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.[2]

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Milieu de culture

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Autres remarques

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