HeLa S3

87110901, human cervix, Epithelial

• Synonyme(s) : HeLa-S3 Cells, HeLa/S3 Cells, HeLaS3 Cells, S3 HeLa Cells
• Code UNSPSC : 41106514

Propriétés

• product name: HeLa S3, 87110901
• Source biologique: human cervix
• Mode de croissance: Suspension
• Caryotype: Modal no. 68
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human cervix carcinoma

Description de la lignée cellulaire

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

Application

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Milieu de culture

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

Autres remarques

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