CV-1

87032605, African green monkey kidney, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: CV-1, from African green monkey kidney, 87032605, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Source biologique: African green monkey kidney
• Mode de croissance: Adherent
• Caryotype: 2n = 60, pseudodiploid
• Morphologie: Fibroblast
• Récepteurs: Not specified

Description

Origine de la lignée cellulaire

Monkey African Green kidney fibroblast

Description de la lignée cellulaire

Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.

Milieu de culture

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C.

Autres remarques

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