NRK-49F

86101301, rat kidney, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: NRK-49F, 86101301
• Source biologique: rat kidney
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat kidney fibroblast

Description de la lignée cellulaire

Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.

Application

Contact inhibition and transformation studies

Milieu de culture

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells should be maintained sub-confluent, otherwise they will transform.

Autres remarques

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