TA3 Hauschka

85061102, mouse breast, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: TA3 Hauschka, 85061102
• Source biologique: mouse breast
• Mode de croissance: Adherent
• Caryotype: 2n = 41
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse mammary carcinoma

Description de la lignée cellulaire

In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.

Application

Tumourigenicity studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.

Autres remarques

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