DAUDI Cell Line human

85011437, human blood, Lymphoblast

• Synonyme(s) : DAUDI Cell Culture, Human DAUDI Cells
• Code UNSPSC : 41106514

Propriétés

• product name: DAUDI Cell Line human, 85011437, African-American Burkitt′s lymphoma, from human blood
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: 2n = 46, diploid
• Morphologie: Lymphoblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Burkitt′s lymphoma

Description de la lignée cellulaire

Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Application

Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth is slow on recovery from frozen. Resuscitate using 20% FBS and keep culture flask standing up until exponential growth is established.

Autres remarques

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