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Principaux documents

C8218

Sigma-Aldrich

Monoclonal Anti-Cdk4 antibody produced in mouse

clone DCS-31, ascites fluid

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

DCS-31, monoclonal

Poids mol.

antigen 33 kDa

Contient

15 mM sodium azide

Espèces réactives

rat, mouse, human

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:1,000 using a cultured human tumor cell line extract

Catégories apparentées

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1 of 4

Cet article
C7464C4710P7962
clone

DCS-31, monoclonal

clone

DCS-6, monoclonal

clone

CY-A1, monoclonal

clone

PSTAIR, monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

species reactivity

rat, mouse, human

species reactivity

rat, mouse, human, monkey

species reactivity

mouse, human, monkey, bovine

species reactivity

mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Description générale

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.

Spécificité

The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.

Immunogène

recombinant human Cdk4 protein

Application

Monoclonal Anti-Cdk4 antibody was used in:
  • immunoprecipitation of human melanoma cell line.
  • immunofluorescence of fibrosarcoma cells.
  • western blot analysis
  • immunofluorescence[1]
  • immunohistochemistry[2]

Actions biochimiques/physiologiques

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Adam Z Oskowitz et al.
The international journal of biochemistry & cell biology, 43(11), 1563-1572 (2011-07-29)
Recently we demonstrated that the miRNA regulate human mesenchymal stem cells (hMSCs) differentiation. To determine the role of the miRNA pathway in hMSCs proliferation, Drosha and Dicer knockdown hMSCs were generated using a lentiviral based tetracycline inducible shRNA. hMSCs with
HMGA2 is the partner of MDM2 in well-differentiated and dedifferentiated liposarcomas whereas CDK4 belongs to a distinct inconsistent amplicon
Italiano A, et al.
International Journal of Cancer. Journal International Du Cancer, 122(10), 2233-2241 (2008)
V Cecchinato et al.
Leukemia research, 32(5), 791-797 (2007-10-30)
T acute lymphoblastic leukemia cell lines treated with hexamethylene bisacetamide (HMBA) undergo a delay in cell cycle progression and increase susceptibility to apoptosis, although they never overcome the differentiation block. In accordance with changes in cell cycle and apoptosis, transitory
Therese M Becker et al.
International journal of cancer, 117(4), 569-573 (2005-06-10)
Melanoma-associated germline mutations affecting the tumor suppressor and cyclin-dependent kinase (CDK) inhibitor, CDKN2A/p16INK4a, have been identified in over 100 melanoma-prone families worldwide. To predict the melanoma risk for carriers of specific mutations, mutant p16INK4a can be tested in biochemical and
J Pines et al.
The Journal of cell biology, 115(1), 1-17 (1991-10-01)
We have used immunofluorescence staining to study the subcellular distribution of cyclin A and B1 during the somatic cell cycle. In both primary human fibroblasts and in epithelial tumor cells, we find that cyclin A is predominantly nuclear from S

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