C5753

Chlorophyll a

from spinach

• Formule empirique (notation de Hill): C₅₅H₇₂MgN₄O₅
• Numéro CAS: 479-61-8
• Poids moléculaire : 893.49
• Numéro Beilstein : 3586237
• Numéro CE : 207-536-6
• Numéro MDL: MFCD00079050
• Code UNSPSC : 12171506
• ID de substance PubChem : 24892803
• Nomenclature NACRES : NA.47

Propriétés

• Source biologique: spinach
• Niveau de qualité: 200
• Pureté: ≥85% (HPLC)
• Forme: powder
• Impuretés: chlorophyll b, essentially free
• ε (coefficient d'extinction): 10.6-11.8 × 10⁴ at 428 nm in diethyl ether (vs. 11.17 × 10⁴, lit.); 8.2-9.1 × 10⁴ at 662 nm in diethyl ether (vs. 8.63 × 10⁴, lit.)
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Chaîne SMILES: CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67
• InChI: 1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1
• Clé InChI: ATNHDLDRLWWWCB-AENOIHSZSA-M

Description

Description générale

Chlorophyll a is an important light-harvesting, green or blue color pigment present in the photosynthetic membranes of the chloroplasts in plant cells. It functions as an antenna pigment in photosystems I and II.

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).

Chlorophyll a has been used:

• to study the variation in carotenoids composition with changing colors and maturation in soybean seeds
• to examine the use of chlorophyll distribution maps obtained from hyperspectral imaging for the non-destructive diagnosis of Nitrogen deficiency
• as a standard to determine the concentration of the analyte by fluorescence spectrophotometric technique in the water sample collected from the yellow river
• as a standard to quantify the amount of chlorophyllous pigments gathered from algae by high performance liquid chromatographic (HPLC) technique
• in the identification of pheophytin a from environmentally friendly cultivated green peppers
• to sense different nitric oxide (NO) species

Actions biochimiques/physiologiques

Chlorophyll a binds to the protein molecules CP47 and CP43, forming the inner antenna of the reaction center of Photosystem II. In photosystem I, the binding sites of chlorophyll a includes the polypeptides spread across the membranes. Scarcity in formation of chlorophyll and a low chlorophyll density can lead to deficiency of nitrogen in plants leaves.

Conditionnement

Sealed ampule.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)