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C5024

Sigma-Aldrich

Anhydrase carbonique from bovine erythrocytes

non-denaturing PAGE marker

Synonyme(s) :

Déshydratase carbonique, Hydrolase de carbonate

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About This Item

Numéro CAS:
9001-03-0
Numéro de classification (Commission des enzymes):
4.2.1.1 (BRENDA, IUBMB)
Numéro CE :
232-576-6
Numéro MDL:
MFCD00081467
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Niveau de qualité

200

Forme

lyophilized powder

Poids mol.

~29 kDa

Adéquation

suitable for marker for electrophoresis (non-denaturing PAGE)

Température de stockage

−20°C

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Application

This product is used as a marker to determine the molecular mass of an unknown protein in non-denaturing electrophoresis systems. In such systems, proteins often retain their biological activity, thus allowing in-gel detection using specific substrate stains. This technique also facilitates the recovery of the active protein. The product yields 3 bands.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Bernardo V Alvarez et al.
BMC cardiovascular disorders, 13, 2-2 (2013-01-10)
Carbonic anhydrase enzymes (CA) catalyze the reversible hydration of carbon dioxide to bicarbonate in mammalian cells. Trans-membrane transport of CA-produced bicarbonate contributes significantly to cellular pH regulation. A body of evidence implicates pH-regulatory processes in the hypertrophic growth pathway characteristic
Peter Burghout et al.
Journal of bacteriology, 195(7), 1573-1582 (2013-01-29)
Although carbon dioxide (CO2) is known to be essential for Streptococcus pneumoniae growth, it is poorly understood how this respiratory tract pathogen adapts to the large changes in environmental CO2 levels it encounters during transmission, host colonization, and disease. To
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
Romualdo Talento et al.
Ultrastructural pathology, 37(1), 70-76 (2013-02-07)
Despite progress in the classification of renal cell carcinomas (RCC), a subset of these carcinomas remains unclassified (RCC-U). Patients with RCC-U usually present at a late stage and have a poor prognosis. Several studies have attempted to extract new classifications
Wolfgang Maret
Advances in nutrition (Bethesda, Md.), 4(1), 82-91 (2013-01-16)
The nutritional essentiality of zinc for the growth of living organisms had been recognized long before zinc biochemistry began with the discovery of zinc in carbonic anhydrase in 1939. Painstaking analytical work then demonstrated the presence of zinc as a
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