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Merck
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Key Documents

C4562

Sigma-Aldrich

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

clone hHCD, ascites fluid

Synonyme(s) :

Anti-CDM, Anti-H-CAD, Anti-HCAD, Anti-L-CAD, Anti-LCAD, Anti-NAG22, Anti-h-CD

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About This Item

Numéro MDL:
MFCD00164554
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

hHCD, monoclonal

Contient

15 mM sodium azide

Espèces réactives

sheep, rabbit, human, pig, bovine, mouse

Technique(s)

immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
immunoprecipitation (IP): suitable
western blot: 1:4,000 using human uterus extract

Isotype

IgG1

Numéro d'accès UniProt

Q05682

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CALD1(800)
mouse ... Cald1(109624)

Spécificité

The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.

Immunogène

human uterus smooth muscle extract.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:
  • immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
  • immunoprecipitation
  • western blot at a dilution of 1:4,000 using human uterus extract

Actions biochimiques/physiologiques

Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Melissa F Brereton et al.
PloS one, 8(2), e57451-e57451 (2013-02-26)
Adequate blood flow through placental chorionic plate resistance arteries (CPAs) is necessary for oxygen and nutrient transfer to the fetus and a successful pregnancy. In non-placental vascular smooth muscle cells (SMCs), K(+) channels regulate contraction, vascular tone and blood flow.
E Vardar et al.
Biomaterials, 206, 41-48 (2019-03-30)
Stress urinary incontinence (SUI) is a life changing condition, affecting 20 million women worldwide. In this study, we developed a bioactive, injectable bulking agent that consists of Permacol™ (Medtronic, Switzerland) and recombinant insulin like growth factor-1 conjugated fibrin micro-beads (fib_rIGF-1)
Jennifer M Kleinhenz et al.
PloS one, 10(10), e0139756-e0139756 (2015-10-10)
Activation of the nuclear hormone receptor, PPARγ, with pharmacological agonists promotes a contractile vascular smooth muscle cell phenotype and reduces oxidative stress and cell proliferation, particularly under pathological conditions including vascular injury, restenosis, and atherosclerosis. However, pharmacological agonists activate both
M G Frid et al.
Developmental biology, 153(2), 185-193 (1992-10-01)
Expression of the regulatory contractile proteins, heavy caldesmon (h-caldesmon) and calponin was studied in human aortic smooth muscle cells (SMCs) during development and compared with the expression of alpha-SM-actin and smooth muscle-myosin heavy chain (SM-MHCs). For this study, novel monoclonal
Min-Chien Tsai et al.
Circulation research, 105(5), 471-480 (2009-07-25)
Phenotypic modulation of smooth muscle cells (SMCs), which are located in close proximity to endothelial cells (ECs), is critical in regulating vascular function. The role of flow-induced shear stress in the modulation of SMC phenotype has not been well defined.
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