C-39605

Renal Epithelial Cell Growth Medium 2

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

• Synonyme(s) : Recommended with C-26230
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.71

Propriétés

• Forme: liquid
• Conditionnement: pkg of 1 kit
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Renal Epithelial Cell Growth Medium 2 SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-26230

Application

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 36 ng / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 4 pg / mlTransferrin (recombinant human): 5 µg / ml

Stockage et stabilité

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Informations sur la sécurité

• Pictogrammes: GHS02,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H225,H290,H319
• Conseils de prudence: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 55.4 °F
• Point d'éclair (°C): 13 °C