C3956

Anti-c-Myc antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Myc Tag Polyclonal Antibody, c-Myc Antibody
• Numéro MDL: MFCD00239651
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.56

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Concentration: ~0.5 mg/mL
• Technique(s): immunocytochemistry: 5-10 μg/mL; immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts; indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells; microarray: suitable; western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.
• Numéro d'accès UniProt: P01106
• Température de stockage: −20°C
• Informations sur le gène: human ... MYC(4609)

Description

Description générale

Anti-c-Myc antibody is used for the detection of the c-Myc tag sequence on c-Myc tagged fusion proteins.This polyclonal antibody is produced in rabbit and recognizes the c-Myc sequence at the N-terminus and C-terminus. C3956 is an Affinity purified antibody, increasing sensitivity in most applications.

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. It is located on chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.

Spécificité

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Immunogène

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

Application

Anti-c-Myc antibody has been used in western blotting and immunofluorescence.

Antibody is recommended for use in immunoblotting, immunoprecipitation and immunofluorescence.
.

Actions biochimiques/physiologiques

MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP). The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as transcriptional regulator of gene expression.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)