Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

C-29910

Sigma-Aldrich

Freezing Medium Cryo-SFM

Serum-free medium for cryopreservation, 30 ml

Synonyme(s) :

Cryopreservation medium

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Forme

aqueous solution

Conditionnement

pkg of 30 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Cryo-SFM

Application

Cryo-SFM is a protein-free, defined and animal component-free cryopreservation medium. The optimized formulation is based on methylcellulose, DMSO and other cryoprotectants for the cryopreservation of human and animal primary cells. Cryo-SFM provides excellent results with all types of cells including primary human cells, stem cells and cell lines. Cells frozen in Cryo-SFM exhibit superior viability, attachment and subsequent growth performance after thawing. The medium is the first choice for all types of primary cells and cell lines.

Qualité

All lots of PromoCell Cryo-SFM are subjected to comprehensive quality control tests. Each lot is routinely tested for its performance with primary human cells, for the absence of cytotoxicity, and for physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots of Cryo-SFM have been tested for the absence of microbial contaminants (fungi, bacteria).

Notes préparatoires

Detach adherent cells using the PromoCell DetachKit (C-41200, C-41210, C-41220). Resuspend the cells in cold or room temperature Cryo-SFM at a concentration of 0.1 to 10 million cells/ml. Transfer the cell suspension into cryo-vials, freeze them gradually (-1°C/minute) to -70°C or lower overnight and subsequently store the vials in liquid nitrogen. Note: Each freeze/thaw cycle leads to a loss in proliferation potential and cell number, particularly when working with fragile primary cells. If freezing the cells, please do so at as low a passage number as possible.

Stockage et stabilité

Store Cryo-SFM at 4 to 8°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.

Procédure de repiquage

Click here for more information.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. 

Primary Human Pericytes and Pericyte Growth Media

Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Mammary Epithelial Cell Growth Medium

Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.

Afficher tout

Protocoles

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique