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Key Documents

C-21160

Sigma-Aldrich

Airway Epithelial Cell Growth Medium

Kit including Basal Medium and SupplementPack, 500 ml

Synonyme(s) :

Airway Cell Medium, Epithelial Growth Medium, Growth Medium for Airway Cells

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Forme

liquid

Conditionnement

pkg of 500 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Description générale

Airway Epithelial Cell Growth Medium Kit

Application

Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages. The medium is optimized for primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells.

Recommended for:
Human Nasal Epithelial Cells (HNEpC)
Human Bronchial Epithelial Cells (HBEpC)
Human Tracheal Epithelial Cells (HTEpC)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin, holo (human): 10 µg / mlRetinoic Acid: 0.1 ng / ml

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Pictogrammes

FlameCorrosion
GHS02,GHS05

Mention d'avertissement

Danger

Mentions de danger

H225,H290,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

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