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Merck
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Key Documents

C0356

Sigma-Aldrich

Protein Extraction Reagent Type 4

Synonyme(s) :

Protein extraction solution

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About This Item

Code UNSPSC :
41116012
Nomenclature NACRES :
NA.56

Forme

solid

Niveau de qualité

Solubilité

deionized water: 13.6 g /15 ml at 25 °C, clear to hazy

Description générale

Protein Extraction Reagent Type 4, is a highly premixed, prequalified and effective protein solubilization and extraction reagent composed of urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Application

Protein extraction reagent type 4 is optimized for proteomic studies. Cellular and organelle membrane solubilizing reagent is ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. Reducing and alkylating reagents TBP (T7567) and iodoacetamide (A3221) are recommended for use with this reagent. Protein extraction reagent type 4 is also available as a component of the ProteoPrep® Sample.

Composants

Contains urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Informations légales

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardExclamation markEnvironment

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Meritxell Pérez-Hedo et al.
Proteome science, 10(1), 58-58 (2012-10-02)
Sesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter
Gabriel A Grilo et al.
Journal of molecular and cellular cardiology, 139, 62-74 (2020-01-25)
Age-related remodeling of the heart causes structural and functional changes in the left ventricle (LV) that are associated with a high index of morbidities and mortality worldwide. Some cardiac pathologies in the elderly population vary between genders revealing that cardiac
John McCarthy et al.
Journal of proteome research, 2(3), 239-242 (2003-06-20)
Carbamylation is widely quoted as being a problem in 2-D gel analysis and the associated sample preparation steps. This modification occurs when iso-cyanate, a urea break-down product, covalently modifies lysine residues, thus inducing a change in isoelectric point. Urea is
Roger A Moore et al.
Proteomics, 10(15), 2858-2869 (2010-06-03)
Transmissible spongiform encephalopathies (TSEs) or prion diseases are characterized by the accumulation of an aggregated isoform of the prion protein (PrP). This pathological isoform, termed PrP(Sc), appears to be the primary component of the TSE infectious agent or prion. However
Chawalit Kocharunchitt et al.
Molecular & cellular proteomics : MCP, 11(1), M111-M111 (2011-10-20)
An integrated transcriptomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass chilling in cold air. The response of

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