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A2050

Sigma-Aldrich

Avidin–FITC from egg white

buffered aqueous solution

Synonyme(s) :

Avidin–Fluorescein isothiocyanate

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About This Item

Numéro MDL:
MFCD00130585
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

FITC conjugate

Niveau de qualité

200

Forme

buffered aqueous solution

Ampleur du marquage

3-5.5 mol fluorochrome per mol protein

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL
indirect immunofluorescence: 1:200

Numéro d'accès UniProt

P02701

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

chicken ... AVD(396260)

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Description générale

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites.

Application

Avidin-FITC from egg white has been used in:
  • flow cytometry
  • double immunostaining
  • double immunofluorescence
  • immunnohistochemistry

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Forme physique

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Notes préparatoires

Affinity purified protein.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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