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A1902

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Rabbit Anti-Mouse IgG (whole molecule)−AP

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About This Item

Numéro MDL:
MFCD00162640
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

200

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

mouse

Technique(s)

direct ELISA: 1:1,000
western blot: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

Spécificité

Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Actions biochimiques/physiologiques

IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Forme physique

Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A M Robinson et al.
Biochimica et biophysica acta, 1369(2), 278-286 (1998-04-29)
Immunoliposomes have been prepared using antibodies raised to an antigenic determinant on the cell surface of the oral bacterium Streptococcus oralis (S. oralis) in an investigation of their potential to reduce dental plaque. The N-succinimidyl-S-acetylthioacetate (SATA) derivative of the antibodies
Immunohistochemical Detection of a Unique Protein within Cells of Snakes Having Inclusion Body Disease, a World-Wide Disease Seen in Members of the Families Boidae and Pythonidae
<BIG>Chang LW, et al</BIG>
PLoS ONE, 8, 2013-2013 null
Michael J Abrams et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(26), E3365-E3373 (2015-06-17)
What happens when an animal is injured and loses important structures? Some animals simply heal the wound, whereas others are able to regenerate lost parts. In this study, we report a previously unidentified strategy of self-repair, where moon jellyfish respond
Nadia Belmellat et al.
Frontiers in immunology, 8, 1533-1533 (2017-12-01)
Tumor necrosis factor-alpha (TNF-α) blockade is an effective treatment for rheumatoid arthritis (RA) and other inflammatory diseases, but in patients, it is associated with reduced resistance to the infectious agents Mycobacterium tuberculosis and Listeria monocytogenes, among others. Our goal was
A role for IgG immune complexes during infection with the intracellular pathogen Leishmania
Miles SA, et al.
The Journal of Experimental Medicine, 201(5), 747-747 (2005)
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