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A1271

Sigma-Aldrich

Adenosine 5′-monophosphate–Agarose

lyophilized powder

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About This Item

Numéro MDL:
MFCD04113722
Code UNSPSC :
41106500
eCl@ss :
32160414
ID de substance PubChem :
329770699
Nomenclature NACRES :
NA.56

Source biologique

plant (Sea weed)

Niveau de qualité

200

Forme

lyophilized powder

Ampleur du marquage

1-5 μmol per mL

Matrice

cross-linked 4% beaded agarose

Activation de la matrice

cyanogen bromide

Fixation de matrice

C-8

Espaceur de matrice

9 atoms

Température de stockage

−20°C

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Catégories apparentées

Application

Adenosine 5′-monophosphate Agarose (5′-AMP agarose) has been used in affinity chromatography to isolate β and gamma glutamate decarboxylase, which is important for controlling gamma-aminobutyric acid (GABA) synthesis in brain.

Forme physique

Lyophilized powder stabilized with lactose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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D L Martin
Cellular and molecular neurobiology, 7(3), 237-253 (1987-09-01)
1. Glutamate decarboxylase is a focal point for controlling gamma-aminobutyric acid (GABA) synthesis in brain. Several factors that appear to be important in the regulation of GABA synthesis have been identified by relating studies of purified glutamate decarboxylase to conditions
F James et al.
The Journal of biological chemistry, 270(38), 22344-22350 (1995-09-22)
The plant enzyme S-adenosylmethionine:methionine S-methyltransferase (EC 2.1.1.12, MMT) catalyzes the synthesis of S-methylmethionine. MMT was purified 620-fold to apparent homogeneity from leaves of Wollastonia biflora. The four-step purification included fractionation with polyethylene glycol, affinity chromatography on adenosine-agarose, anion exchange chromatography
S K Lee et al.
FEBS letters, 316(3), 261-263 (1993-02-01)
The mosquito homolog of mammalian DNA polymerase epsilon, formerly known as a proliferating cell nuclear antigen (PCNA)-independent form of DNA polymerase delta, has been purified from mosquito larval extracts. The polymerase epsilon was separated from DNA polymerase alpha by chromatography
C D Murphy et al.
Applied and environmental microbiology, 67(10), 4919-4921 (2001-09-26)
Streptomyces cattleya is unusual in that it produces fluoroacetate and 4-fluorothreonine as secondary metabolites. We now report the isolation of an NAD(+)-dependent fluoroacetaldehyde dehydrogenase from S. cattleya that mediates the oxidation of fluoroacetaldehyde to fluoroacetate. This is the first enzyme
S M Pettit et al.
Clinical chemistry, 27(1), 88-93 (1981-01-01)
We present a method for preparing human liver lactate dehydrogenase (L-lactate:NAD+ oxidoreductase; EC 1.1.1.27) isoenzyme-5 by sequential ion-exchange chromatography, general-ligand (AMP analog) affinity chromatography, and preparative isoelectric focusing. The yield ws 40%, with a 493-fold purification. The final specific activity
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