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94740

Sigma-Aldrich

Vanadyl ribonucleoside complexes

BioReagent, for molecular biology

Synonyme(s) :

Ribonucleoside vanadyl complexes, VRC

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

Gamme de produits

BioReagent

Forme

powder

Adéquation

in accordance for ribonuclease inhibition test

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.

Actions biochimiques/physiologiques

The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.

Produit(s) apparenté(s)

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Zhang X, et al.
Oncology Letters, 8(4), 1693-1696 (2014)
G Rotondo et al.
Nucleic acids research, 24(12), 2377-2386 (1996-06-15)
The pac1+ gene of the fission yeast Schizosaccharomyces pombe is essential for viability and its overexpression induces sterility and suppresses mutations in the pat1+ and snm1+ genes. The pac1+ gene encodes a protein that is structurally similar to RNase III
R Boom et al.
Journal of clinical microbiology, 28(3), 495-503 (1990-03-01)
We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together
Inhibitors of RNases
Sambrook, J. and Russell, D.W. et al.
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)

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