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94149

Sigma-Aldrich

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

Synonyme(s) :

Streptavidin-Atto 647N

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About This Item

Code UNSPSC :
12161900
Nomenclature NACRES :
NA.25

Gamme de produits

BioReagent

Niveau de qualité

100

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorption UV

λ: 650.0-656.0 nm Amax

Adéquation

corresponds for gel electrophoresis
suitable for fluorescence

Température de stockage

−20°C

Description générale

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Application

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Caractéristiques et avantages

Atto 647N- Streptavidin has the following benefits-

  • Highly photostable. Strong absorption.
  • Excellent fluorescent quantum yield.
  • Highly resistant to ozone degradation.
  • Minimal triplet formation.

Remarque sur l'analyse

dye-to-protein ratio ≥1.5

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Spatially resolved two-color diffusion measurements in human skin applied to transdermal liposome penetration
Brewer J, et al.
The Journal of Investigative Dermatology, 133(5), 1260-1268 (2013)
John A Bogovic et al.
PloS one, 15(12), e0236495-e0236495 (2021-01-01)
The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Afficher tous les articles scientifiques apparentés

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Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

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