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Merck
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Key Documents

85L1

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-MX phosphate and fast blue RR salt

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About This Item

Code UNSPSC :
12352106
Nomenclature NACRES :
NA.47

Niveau de qualité

Durée de conservation

Expiry date on the label.

IVD

for in vitro diagnostic use

Application(s)

hematology
histology

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-MX phosphate alkaline solution with fast blue RR salt. The resulting blue, insoluble, granular dye deposit indicates sites of alkaline phosphatase activity.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • MHS1Mayer′s Hematoxylin Solution 100 mLFDS

  • 855Naphthol AS-MX Phosphate Alkaline Solution, 0.25% 20 mLFDS

  • FBS25Fast Blue RR Salt, Preweighed Capsules 10 capsulesFDS

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Lee W Tien et al.
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Chun Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(31), E4439-E4445 (2016-07-21)
There is a growing appreciation for the functional role of matrix mechanics in regulating stem cell self-renewal and differentiation processes. However, it is largely unknown how subcellular, spatial mechanical variations in the local extracellular environment mediate intracellular signal transduction and
Clarissa S Craft et al.
The Journal of biological chemistry, 285(31), 23858-23867 (2010-05-27)
MAGP1 is an extracellular matrix protein that, in vertebrates, is a ubiquitous component of fibrillin-rich microfibrils. We previously reported that aged MAGP1-deficient mice (MAGP1Delta) develop lesions that are the consequence of spontaneous bone fracture. We now present a more defined
Alban J Smith et al.
Scientific reports, 7, 41872-41872 (2017-02-06)
The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling.
Shigeki Ohta et al.
PloS one, 6(1), e16182-e16182 (2011-01-21)
Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation

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