Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

50283

Sigma-Aldrich

Anti-Mouse IgG - Atto 655 antibody produced in goat

~1 mg/mL IgG

Synonyme(s) :

Atto 655 [goat Anti-mouse IgG]

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

100

Produit recombinant

expressed in HEK 293 cells

Conjugué

Atto 655 conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Contient

50% glycerol

Espèces réactives

mouse

Concentration

~1 mg/mL IgG

Technique(s)

immunofluorescence: suitable

Fluorescence

λex 655 nm; λem 677 nm in PBS

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Cat. No. 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

Immunogène

purified mouse IgG

Application

Atto 655-goat anti-Mouse IgG - Atto 655 antibody has been used for immunofluorescence applications.

Conditionnement

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Remarque sur l'analyse

Unconjugated dye ≤5% of total fluorescence.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Conseils de prudence

P305 + P351 + P338

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Anindita Dasgupta et al.
Nature methods, 21(7), 1171-1174 (2024-06-05)
Fluorescence microscopy is limited by photoconversion due to continuous illumination, which results in not only photobleaching but also conversion of fluorescent molecules into species of different spectral properties through photoblueing. Here, we determined different fluorescence parameters of photoconverted products for
Keith Pardee et al.
Cell, 167(1), 248-259 (2016-09-24)
Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs, therapeutic proteins, and other commodities. However, the need for specialized equipment and refrigeration for production and distribution poses a challenge for the delivery of these technologies to
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:

Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique