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Sigma-Aldrich

Atto 532 DOPE

suitable for fluorescence

Synonyme(s) :

1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 532

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About This Item

Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32

Pureté

≥80.0% (HPCE)

Niveau de qualité

100

Fabricant/nom de marque

ATTO-TEC GmbH

Fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Description générale

Atto 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus Atto 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm.
A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.

Atto-Dye Labeled Phospholipids
Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

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Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
STED nanoscopy. A glimpse into the future.
Bianchini, P.; et al.
Cell and Tissue Research, 360(1), 143-150 (2015)
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