27815

Bleu brillant G

250, for microscopy

• Synonyme(s) : Bleu acide 90, Bleu brillant G de Coomassie
• Formule empirique (notation de Hill): C₄₇H₄₈N₃NaO₇S₂
• Numéro CAS: 6104-58-1
• Poids moléculaire : 854.02
• Numéro C.I. (Colour Index): 42655
• Numéro Beilstein : 5230822
• Numéro CE : 228-058-4
• Numéro MDL: MFCD00078482
• Code UNSPSC : 12171500
• ID de substance PubChem : 329752898
• Nomenclature NACRES : NA.26

Propriétés

• Qualité: for microscopy
• Niveau de qualité: 200
• Forme: solid
• Technique(s): microbe id | staining: suitable
• Chaîne SMILES: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
• Clé InChI: RWVGQQGBQSJDQV-UHFFFAOYSA-M

Description

Description générale

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Actions biochimiques/physiologiques

Antagoniste des récepteurs purinergiques P2X7.

Remarque sur l'analyse

λ_max. ∼610 nm; E(1%,1 cm) ∼500-700 (H₂O)

Autres remarques

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Informations légales

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)