08061901
U-373 MG (Uppsala) cell line
Synonyme(s) :
U-373MG Cells, U373-MG Cells, U373MG Cells
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About This Item
Code UNSPSC :
41106514
Produits recommandés
Source biologique
human brain
Niveau de qualité
Forme
liquid
Mode de croissance
Adherent
Caryotype
2n = 46
Morphologie
Pleomorphic/astrocytoid
Récepteurs
PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
cancer
Origine de la lignée cellulaire
Human glioblastoma astrocytoma
Description de la lignée cellulaire
A human glioblastoma astrocytoma derived from a malignant tumour by explant technique. This catalogue entry represents a new deposit of U-373 MG obtained from the originator′s laboratory in Uppsala; it has been designated U-373 MG (Uppsala) to distinguish it from U-373 MG (Sigma Catalogue number 89081403) which is now known to be identical, by Short Tandem Repeat (STR)-PCR profiling, to another glioblastoma cell line, U-251 MG (please read below for more information about this discovery). U-373 MG (Uppsala) has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.
Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′.
Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′.
Application
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).
Actions biochimiques/physiologiques
U-373 MG (Uppsala) is a human glioblastoma astrocytoma derived from a malignant tumour by explant technique.
Profil d'ADN
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8
D16S539: 12,13
D5S818: 12
D7S820: 8.2,12
THO1: 7,9.3
TPOX: 8
vWA: 17,18
CSF1PO: 11,12
D13S317: 8
D16S539: 12,13
D5S818: 12
D7S820: 8.2,12
THO1: 7,9.3
TPOX: 8
vWA: 17,18
Milieu de culture
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Procédure de repiquage
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Autres remarques
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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