PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• Synonyme(s) : Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• Code UNSPSC : 41105323
• Nomenclature NACRES : NA.21

Propriétés

• product name: Tris Buffered Saline, with 0.05% Tween^® 20, pH 8.0, powder, pHast Pack^™
• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry.)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 7.8—8.2
• Solubilité: soluble, clear to hazy, colorless
• Traces de cations: Fe: ≤10 ppm
• Activité étrangère: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Température de stockage: room temp

Description

Description générale

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Application

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

Caractéristiques et avantages

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable