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N2125

Sigma-Aldrich

Naphthol AS-BI phosphate

≥98% purity (HPLC), powder

Synonyme(s) :

7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate

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About This Item

Formule empirique (notation de Hill):
C18H15BrNO6P
Numéro CAS:
1919-91-1
Poids moléculaire :
452.19
Numéro Beilstein :
6668526
Numéro CE :
217-645-0
Numéro MDL:
MFCD00004058
Code UNSPSC :
12171500
ID de substance PubChem :
24897552
Nomenclature NACRES :
NA.47

product name

Naphthol AS-BI phosphate, ≥98% (HPLC), Technical grade

Niveau de qualité

200

Pureté

≥98% (HPLC)

Forme

powder

Technique(s)

microbe id | staining: suitable

Couleur

white to light yellow

Solubilité

methanol: 50 mg/mL

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

−20°C

Chaîne SMILES 

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

Clé InChI

HUXIAXQSTATULQ-UHFFFAOYSA-N

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Description générale

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.

Application

Naphthol AS-BI phosphate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Actions biochimiques/physiologiques

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrats

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCK2119
BCCD2606
BCBS3419V
BCBS3419
BCBH1155

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.
P J Stoward et al.
Histochemistry, 71(3), 405-431 (1981-01-01)
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
Anthony J Janckila et al.
Clinica chimica acta; international journal of clinical chemistry, 347(1-2), 157-167 (2004-08-18)
Our purpose was to develop a specific immunoassay for tartrate-resistant acid phosphatase (TRACP) 5b using naphthol ASBI phosphate (N-ASBI-P) as a selective substrate for isoform 5b and heparin as a selective inhibitor of isoform 5a. Serum TRACP 5a and 5b
A M Boesen
Scandinavian journal of haematology, 32(3), 245-252 (1984-03-01)
The subcellular localization of acid phosphatase (AcP) in various immunologically-defined neoplastic lymphoid cells including hairy cells was investigated by electron microscopy. 2 substrates, naphthol-AS-BI-phosphoric acid (naphthol-AS-BI-P) and sodium beta-glycerophosphate, were compared. By incubation in naphthol-AS-BI-P containing medium, the reaction product
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