94635

Gram′s safranin solution

for microscopy

• Synonyme(s) : Safranin T solution
• Formule empirique (notation de Hill): C₂₀H₁₉ClN₄
• Numéro CAS: 477-73-6
• Poids moléculaire : 350.84
• Numéro Beilstein : 3924099
• Numéro MDL: MFCD00011759
• Code UNSPSC : 12171500
• ID de substance PubChem : 329770368
• Nomenclature NACRES : MA.02

Propriétés

• Qualité: for microscopy
• Niveau de qualité: 100
• Forme: liquid
• Durée de conservation: limited shelf life, expiry date on the label
• Composition: ethanol, 10%
• Technique(s): microbe id | staining: suitable
• Couleur: red to very dark red
• Indice de réfraction: n20/D 1.339
• Solubilité: water: soluble at 20 °C
• Densité: 0.986 g/mL at 20 °C
• εmax: 4.0 at 532 nm in 50% ethanol
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• Clé InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Description

Description générale

Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.

Application

Gram’s safranin solution has been used in the following applications:

• as a non-toxic replacement for crystal violet for the quantification of biofilm formation
• to develop a hyaluronic acid visualization method
• to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole

Caractéristiques et avantages

• Ready-to-use solution.
• Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems.

Principe

Gram staining enables the differentiation of bacteria into Gram-positive and Gram-negative. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with crystal violet, and then with iodine solution, which forms a dye-iodine complex. This complex is retained in the multi-layer mureine structure of the cell wall of Gram-positive bacteria after decolorization, imparting a blue-violet color. The cell wall of Gram-negative bacteria comprises a single-layered murein structure that does not retain the dye-iodine complex, allowing the uptake of safranine counterstain, and imparting a pink-red color.

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P210 - P233 - P240 - P241 - P242 - P243
• Classification des risques: Flam. Liq. 3
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 114.8 °F
• Point d'éclair (°C): 46 °C
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter