67515

STEC Millichrome^™ plus Supplement

suitable for microbiology

• Synonyme(s) : Shiga-Toxin producing E. coli (STEC) Chromogenic supplement
• Code UNSPSC : 41171614
• Nomenclature NACRES : NA.74

Propriétés

• Stérilité: aseptically filled
• Niveau de qualité: 200
• Forme: dry powder
• Durée de conservation: limited shelf life, expiry date on the label
• Technique(s): microbiological culture: suitable
• Solubilité: sterile water: 1 (vial/10ml)
• Application(s): food and beverages; microbiology
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Adéquation: selective for bacteria: Escherichia coli (Shiga Toxin-STEC

Description

Description générale

STEC Millichrome^™ plus Supplement is a selective media supplement for the detection of E. coli (STEC). The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli (STEC).Millichrome^™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, each vial sufficient for 1 liter of final media. One vial of the supplement should be rehydrated with 10 mL of sterile water and then added to 1 liter of melted STEC Millichrome^™ plus Agar base cooled at 45-50 °C.

Application

STEC Millichrome^™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of E. coli (STEC) from food, animal feed, and environmental samples.

Conditionnement

5 vials (1 vial is sufficient for 1 L of final media)

Notes préparatoires

• Aseptically rehydrate 1 vial with 10 mL of sterile water.
• Swirl well until complete dissolution.
• Add 1 vial rehydrated solution to 1L STEC Millichrome^® plus Agar base cooled at 45-50 °C.
• Swirl gently to homogenize.

Remarque sur l'analyse

The Shiga toxins E. coli is detected by a characteristic mauve color after only 24 h at an optimal growth temperature of 37°C, while most other Enterobacteriaceae form Colorless, blue, or inhibited after incubation at the same temperature

Informations légales

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302
• Conseils de prudence: P264 - P270 - P301 + P312 - P501
• Classification des risques: Acute Tox. 4 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3