318752

Bromothymol Blue sodium salt solution

0.04 wt. % in H₂O

• Formule empirique (notation de Hill): C₂₇H₂₇Br₂NaO₅S
• Numéro CAS: 34722-90-2
• Poids moléculaire : 646.36
• Numéro MDL: MFCD00077263
• Code UNSPSC : 12171500
• ID de substance PubChem : 24859144
• Nomenclature NACRES : NA.47

Propriétés

• Forme: liquid
• Niveau de qualité: 200
• Concentration: 0.04 wt. % in H₂O
• pH: 6.0-7.6, yellow to blue
• Densité: 0.99 g/mL at 25 °C (lit.)
• λmax: 615 nm
• ε (coefficient d'extinction): ≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L; ≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
• InChI: 1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
• Clé InChI: NMKFVGALBGZKGW-FKWCIMQXSA-M

Description

Description générale

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Actions biochimiques/physiologiques

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)