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10838039001

Roche

Fibronectine

from human plasma

Synonyme(s) :

Fibronectin

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About This Item

Numéro MDL:
MFCD00131062
Code UNSPSC :
12352207

Source biologique

human plasma

Niveau de qualité

100

Stérilité

non-sterile; 0.2 μm filtered

Pureté

95% (SDS-PAGE)

Forme

lyophilized (clear, colorless solution after reconstitution)

Poids mol.

440 kDa

Conditionnement

pkg of 5 mg

Fabricant/nom de marque

Roche

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Numéro d'accès UniProt

P02751

Température de stockage

2-8°C

Informations sur le gène

human ... FN1(2335)

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Catégories apparentées

Description générale

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Spécificité

Active on most mammalian cells

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Méthode d'analyse

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Forme physique

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Notes préparatoires

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Daniella Penko et al.
Cell transplantation, 24(1), 37-48 (2013-09-28)
The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve the engraftment of transplanted pancreatic islets. In addition, EPCs may
Hao Wang et al.
Microsystems & nanoengineering, 8, 49-49 (2022-05-14)
Cardiovascular disease is the number one cause of death in humans. Therefore, cardiotoxicity is one of the most important adverse effects assessed by arrhythmia recognition in drug development. Recently, cell-based techniques developed for arrhythmia recognition primarily employ linear methods such
Daniella Penko et al.
Islets, 3(3), 73-79 (2011-04-12)
Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for
Ana Lonic et al.
STAR protocols, 3(2), 101305-101305 (2022-05-03)
Previously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling
Tomofumi Yamamoto et al.
Blood advances, 6(8), 2480-2495 (2022-01-04)
Multiple myeloma (MM) is a hematopoietic malignancy whose prognosis has improved with the development of new agents such as lenalidomide over the last decade. However, long-term exposure to drugs induces the acquisition of resistance by MM cells and leads to treatment
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Articles

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Protocoles

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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