10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Synonyme(s) :
lambda dna
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About This Item
Code UNSPSC :
41106310
Produits recommandés
Niveau de qualité
Forme
solution
Poids mol.
32,300 kDa
Conditionnement
pkg of 1 mL
Fabricant/nom de marque
Roche
Conditions de stockage
avoid repeated freeze/thaw cycles
Concentration
250 μg/mL
Impuretés
RNA, none detected
nuclease, none detected
Couleur
colorless
Solubilité
water: miscible
Conditions d'expédition
dry ice
Température de stockage
−20°C (−15°C to −25°C)
Description générale
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Application
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Qualité
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Séquence
Chain Length 48,502 bp
Forme physique
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Stockage et stabilité
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
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