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10107921001

Roche

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

Synonyme(s) :

s7 nuclease

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About This Item

Code UNSPSC :
12352204

Source biologique

Staphylococcus aureus

Niveau de qualité

100

Forme

lyophilized

Activité spécifique

~15,000 U/mg

Conditionnement

pkg of 15,000 U

Fabricant/nom de marque

Roche

pH optimal

7.5

Conditions d'expédition

wet ice

Catégories apparentées

Application

  • Removal of endogenous RNA from in vitro translation systems
  • RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

Actions biochimiques/physiologiques

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

Définition de l'unité

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.

Stockage et stabilité

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Exclamation markHealth hazard
GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H315,H317,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Reimo Zoschke et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), E1678-E1687 (2015-03-17)
Chloroplast genomes encode ∼ 37 proteins that integrate into the thylakoid membrane. The mechanisms that target these proteins to the membrane are largely unexplored. We used ribosome profiling to provide a comprehensive, high-resolution map of ribosome positions on chloroplast mRNAs
Ariane Lismer et al.
Nucleic acids research, 48(20), 11380-11393 (2020-10-18)
Advancing the molecular knowledge surrounding fertility and inheritance has become critical given the halving of sperm counts in the last 40 years, and the rise in complex disease which cannot be explained by genetics alone. The connection between both these
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
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