03359123001
Roche
Alkaline Phosphatase recombinant, highly active
EIA Grade, from Pichia Pastoris
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About This Item
Produits recommandés
Niveau de qualité
Pureté
≥95% (HPLC)
Forme
buffered aqueous solution (ready-to-use, 20 mg/ml)
Activité spécifique
≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
Conditionnement
pkg of 10 mg (500 μl)
Fabricant/nom de marque
Roche
Concentration
20 mg/mL (ready-to-use)
pH optimal
8.0(Stability)
9.8(Activity)
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.
The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
Spécificité
Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.
Application
Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.
Actions biochimiques/physiologiques
Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.
Caractéristiques
≥7,000 U/mg (+37°C; pNPP; DEA)
Forme physique
Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.
Notes préparatoires
Activator: Mg2-, Co2- and Mn2-ions
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
Certificats d'analyse (COA)
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