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Key Documents

SCT282

Sigma-Aldrich

BioTracker™ Mito Membrane Potential Live Cell Detection Kit

Human

Synonyme(s) :

MMP live cell probe, mitochondrial membrane potential probe

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NB.24

product name

BioTracker™ Mito Membrane Potential Live Cell Detection Kit,

Source biologique

human

Niveau de qualité

100

Conditionnement

vial of 1 kit

Fabricant/nom de marque

Millipore

Technique(s)

cell culture | stem cell: suitable
flow cytometry: suitable

Conditions d'expédition

2-8°C

Température de stockage

2-8°C

Description générale

The SCT282 Mito Membrane Potential Live Cell Detection Kit overcomes the limitations of reagents likeJC-1 dye, TMRE, and TMRM widely used to monitor mitochondrial embrane potential (MMP). These existing reagents often exhibit low photostability and poor retention after aldehyde fixation. Such limitations result in poor reproducibility of experiments. SCT282 demonstrates remarkable retention and photostability that enable reliable, reproducible monitoring of MMP.

Caractéristiques et avantages

This live cell probe is highly sensitive to changes in mitochondrial membrane potential.

Description de la cible

Mitochondria are important organelles that utilize oxygen to synthesize ATP, producing the necessary energy for living cells to function and survive. Decreased mitochondrial activity and mitochondrial dysfunction are associated with cancer, aging, and neurodegenerative diseases such as Alzheimer′s and Parkinson′s diseases (AD and PD). Mitochondrial membrane potential (MMP) has therefore been widely studied in the context of health and disease where mitochondrial function is a key indicator.References1) K. F. Ferri, et al., J. Exp. Med., 2000, 192, 1081.2) N. Matsuda, et al., J. Cell Biol., 2010, 189, 211.3) J. L. Wang, et al., PNAS, 2000, 97, 7124.

Stockage et stabilité

Store BioTracker™ Mito Membrane Potential Live Cell Detection Kit at -20 °C, protected from light.

Autres remarques

SCT282 is a live cell probe, but is also amenable to fixation after staining.Diluting BioTracker™ Mito Membrane Potential Live Cell Detection Kit 1:1000 is recommended, but dilution may vary from 1:500 to 1:2000, depending on the end user application. Dilute the 10x Imaging Buffer (1:10) in double-deionized water.Dilute the MT-1 Dye (1:1000) in the cell culture medium to prepare working solution, and use the same day.

Informations légales

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

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