MABS1904
Anti-RAMP1 Antibody, clone 4F8.1
clone 4F8.1, from mouse
Synonyme(s) :
Receptor activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
4F8.1, monoclonal
Espèces réactives
human
Conditionnement
antibody small pack of 25 μg
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG2bκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RAMP1(10267)
Description générale
Receptor activity-modifying protein 1 (UniProt: O60894; also known as Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, RAMP1) is encoded by the RAMP1 gene (Gene ID: 10267) in human. RAMP1 is a single-pass type I membrane protein that is involved in the transports of calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane and acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. RAMP1 lacks glycosylation sites and does not migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin. It is expressed in many tissues including the uterus, bladder, brain, pancreas, retina, and gastro-intestinal tract. It is synthesized with a signal peptide (aa 1-26), which is cleaved to generate the mature form. Mature RAMP1 has an extracellular domain (aa 27-117); a transmembrane domain (aa 118-138); and a short cytoplasmic tail (aa 139-148). RAMP1 is reported to acts as a vasodilation factor and mice deficient in RAMP1 exhibit inflammatory responses with a transient, but significant, increase in serum calcitonin-gene-related peptide levels and pro-inflammatory cytokines. (Ref.: Nakayama, T., et al. (2017). J. Hum. Hypertens. 31(5); 361-365).
Spécificité
Clone 4F8.1 specifically detects Receptor activity-modifying protein 1 (RAMP1) in human cells. It targets an epitope within 91 amino acids from the internal region.
Immunogène
GST/His-tagged recombinant fragment corresponding to 91 amino acids from the internal region of human Receptor activity-modifying protein 1 (RAMP1).
Application
Anti-RAMP1, clone 4F8.1, Cat. No. MABS1904, is a mouse monoclonal antibody that detects Receptor activity-modifying protein 1 (RAMP1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RAMP1 in human uterus and human colon tissue sections.
Research Category
Signaling
Signaling
Qualité
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAMP1 in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAMP1 in human brain tissue lysate.
Description de la cible
~17 kDa observed; 16.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Camille Cohen et al.
Nature communications, 15(1), 743-743 (2024-01-26)
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