MABF39-I
Anti-DC-STAMP Antibody, clone 1A2
clone 1A2, from mouse
Synonyme(s) :
Dendritic cell-specific transmembrane protein, DC-STAMP, Dendrocyte-expressed seven transmembrane protein, FIND, hDC-STAMP, IL-four-induced protein, Transmembrane 7 superfamily member 4
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.45
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
1A2, monoclonal
Espèces réactives
mouse, human
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... DCSTAMP (81501)
Description générale
Dendritic cell-specific transmembrane protein (UniProt Q9H295; also known as DC-STAMP, Dendrocyte-expressed seven transmembrane protein, FIND, hDC-STAMP, IL-four-induced protein, Transmembrane 7 superfamily member 4) is encoded by the DCSTAMP (also known as TM7SF4) gene (Gene ID 81501) in human. DC-STAMP is a six-transmembrane protein essential for cell-to-cell fusion to form multinucleated osteoclasts (OCs) during osteoclastogenesis. DC-STAMP expression is upregulated among osteoclast precursor (OCP) cells upon exposure to OC-promoting cytokines, such as receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL), and Dcstamp-knockout (KO) mice have few multinucleated TRAP+ OCs and increased bone mass. On the other hand, DC-STAMP overexpression in transgenic (Tg) mice causes accelerated cell-to-cell fusion during OCP differentiation and enhanced bone resorption. DC-STAMP is a six-trasmembrane (a.a. 35-55, 58-78. 98-118, 210-230, 293-313, 377-397) protein, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-34, 398-470). The C-terminal cytoplasmic tail of DC-STAMP contains an immunoreceptor tyrosine-based inhibitory motif or ITIM sequence (407-SFYPSV-412) that, when phosphorylated on the tyrosine residue, recruits SHP-1. DC-STAMP neutralizing antibody blocks OC formation in vitro and abolishes cellular DC-STAMP and SHP-1 tyrosine phosphorylation.
Spécificité
Clone 1A2 recognizes an epitope conserved between human and murine DC-STAMP in the third extracellular domain.
Immunogène
Epitope: The third extracellular domain.
KLH-conjugated linear peptide corresponding a sequence from the third extracellular domain of human DC-STAMP.
Application
Anti-DC-STAMP Antibody, clone 1A2 is an antibody against DC-STAMP for use in Western Blotting, Flow Cytometry, Immunocytochemistry, Immunoprecipitation.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Osteobiology
Osteobiology
Western Blotting Analysis: 4.0 µg/mL from a representative lot detected DC-STAMP in 10 µg of thymus lysates from wild-type, but not Dcstamp-knockout mice.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DC-STAMP in thymus and spleen lysates from wild-type mice, and greatly reduced DC-STAMP in thymus and spleen lysates from Dcstamp-knockout mice (Courtesy of Grace Chiu, Ph.D., University of Rochester Medical Center, NY, USA).
Western Blotting Analysis: A representative lot detected the ~106 kDa dimeric and the ~53 kDa monomeric DC-STAMP band by Western blotting under non-denatured and denatured condition, respectively, following DC-STAMP immunoprecipitation using murine RAW 264.7 macrophage lysate (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Flow Cytometry Analysis: A representative lot detected DC-STAMP-positive lymphoctes and monocytes in purified human PBMCs (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Flow Cytometry Analysis: A representative lot detected DC-STAMP surface expression on murine RAW 264.7 macrophages and murine bone marrow-derived CD11b+ monocytes. DC-STAMP is expressed on osteoclast precursor (OCP) cells as a dimer, which is efficiently detected by flow cytometry using clone 1A2 (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Function Analysis: A representative lot inhibited RANKL & M-CSF treatment-induced osteoclasts (OC) formation in human PBMCs & monocytes cultures (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Function Analysis: A representative lot inhibited RANKL treatment-induced osteoclasts (OC) formation in RAW 264.7 and murine bone marrow macrophages cultures (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunohistochemistry Analysis: A representative lot detected DC-STAMP expressionon in multinucleated ‘osteoclast-like’ giant cells in human giant cell tumor of bone (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected DC-STAMP-positive human PBMCs using 10% NBF-fixed, paraffin-embedded cell preparation (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected differential DC-STAMP intracellular localizations by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% saponin-permeabilized murine bone marrow macrophages at different time points during osteoclastogenesis upon RANKL treatment (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the lysates of human monocytes (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the membrane extracts of murine RAW 264.7 macrophages (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DC-STAMP in thymus and spleen lysates from wild-type mice, and greatly reduced DC-STAMP in thymus and spleen lysates from Dcstamp-knockout mice (Courtesy of Grace Chiu, Ph.D., University of Rochester Medical Center, NY, USA).
Western Blotting Analysis: A representative lot detected the ~106 kDa dimeric and the ~53 kDa monomeric DC-STAMP band by Western blotting under non-denatured and denatured condition, respectively, following DC-STAMP immunoprecipitation using murine RAW 264.7 macrophage lysate (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Flow Cytometry Analysis: A representative lot detected DC-STAMP-positive lymphoctes and monocytes in purified human PBMCs (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Flow Cytometry Analysis: A representative lot detected DC-STAMP surface expression on murine RAW 264.7 macrophages and murine bone marrow-derived CD11b+ monocytes. DC-STAMP is expressed on osteoclast precursor (OCP) cells as a dimer, which is efficiently detected by flow cytometry using clone 1A2 (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Function Analysis: A representative lot inhibited RANKL & M-CSF treatment-induced osteoclasts (OC) formation in human PBMCs & monocytes cultures (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Function Analysis: A representative lot inhibited RANKL treatment-induced osteoclasts (OC) formation in RAW 264.7 and murine bone marrow macrophages cultures (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunohistochemistry Analysis: A representative lot detected DC-STAMP expressionon in multinucleated ‘osteoclast-like’ giant cells in human giant cell tumor of bone (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected DC-STAMP-positive human PBMCs using 10% NBF-fixed, paraffin-embedded cell preparation (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunocytochemistry Analysis: A representative lot detected differential DC-STAMP intracellular localizations by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% saponin-permeabilized murine bone marrow macrophages at different time points during osteoclastogenesis upon RANKL treatment (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the lysates of human monocytes (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92).
Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the membrane extracts of murine RAW 264.7 macrophages (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83).
Qualité
Evaluated by Western Blotting in mouse thymus lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected DC-STAMP in 10 µg of mouse thymus lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected DC-STAMP in 10 µg of mouse thymus lysate.
Description de la cible
~58 kDa observed. Target band size appears larger than the calculated molecular weights of 53.39 kDa (human) and 53.88 kDa (mouse) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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DC-STAMP is a multi-pass transmembrane protein essential for cell-cell fusion between osteoclast precursors during osteoclast (OC) development. DC-STAMP-/- mice have mild osteopetrosis and form mononuclear cells with limited resorption capacity. The identification of an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) on
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